E-ISSN: 2587-0831 ISSN: 2587-0831
Problems In Determining Her2 Status In Breast Carcinoma
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Original Article
VOLUME: 11 ISSUE: 1
P: 10-16
January 2015

Problems In Determining Her2 Status In Breast Carcinoma

Eur J Breast Health 2015;11(1):10-16
DOI: 10.5152/tjbh.2014.2103
Emel Ebru Pala
Ümit Bayol
Alp Özgüzer
Ülkü Küçük
Çağlar Yıldız Akdeniz
Özlem Sezer
1. İzmir Tepecik Eğitim ve Araştırma Hastanesi, Patoloji Kliniği, İzmir, Türkiye
No information available.
No information available
Received Date: 12.06.2014
Accepted Date: 14.10.2014
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ABSTRACT

Objective:

Human epidermal growth factor receptor 2 (HER2) oncoprotein is overexpressed in 15-25% of breast carcinomas and associated with poor outcome. Assessment of HER2 status accurately is important to select patients who will benefit from targeted therapy.

Materials and Methods:

In this study immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were used to determine the HER2 status in 308 breast carcinoma cases of which 129 were consultation. The major problems in determining HER2 status and the reasons of discordant results between methods were discussed.

Results:

HER2 expression was (-) in 124, (+) in 29, (++) in 92, (+++) in 63 cases. 25 of 76 cases consulted as (++) were evaluated as (++) and 15 of 35 cases consulted as (+++) were evaluated as (+++). HER2 amplification was found in 88 (28.6%) of 308 cases by FISH. 3 of 124 (-), 1 of 29 (+), 22 of 92 (++), 62 of 63 (+++) cases were amplified by FISH. The relation between HER2 expression and amplification was statistically significant (p<0.001). Centromere 17 (CEN 17) region amplification was noted in 11 cases of which 2 were (+++), 9 were (++). 6 of the 11 cases showed focal low level, 1 of them showed diffuse high level amplification.

Conclusion:

The concordance rate between IHC (+++) cases and FISH was 95.4% for consultation cases, 100% for our cases. The final concordance rate for both case groups was 98.4%. The possible reasons of discrepancy were triple negativity, preanalytical and analytical procedures of consultation cases and trucut samples.